Their Sf9 cell line comes from the fall armyworm and is … I am working on sf9 cell line that is maintained on Grace's Insect media. To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum (Sigma cat# F4135) to a final concentration of 10%. The National Cancer Institute (NCI) seeks licensing partners for a novel modified insect cell line, Sf9-ET, that can quickly and efficiently determine baculovirus titers during the expression of recombinant proteins from a baculovirus-based protein expression system. Most conventional flu vaccines consist of whole, killed flu viruses or viral fragments that contain several proteins, not just hemagglutinin (though one vaccine uses a live, weakened virus). A few insect cell lines are, in routine industrial processes, designed to produce proteins of biomedical significance. Originally derived from the Spodoptera frugiperda (fall armyworm) moth and adapted for cell culture. Most cell lines are adherent cells, which grow only on the surfaces of culture vessels. 1 Introduction Cell Lines Introduction This manual is included with Sf9, Sf21, and High FiveŽ insect cells and provides general information about the growth and maintenance of insect cell cultures. Baculoviruses infect a few insect species and are commonly found on green vegetables but do not grown in mammalian cells, according to previous reports. Modifications to the host insect cell line, for example by including the anti-apoptotic gene Bcl-2, may limit the cytopathic effects of the baculovirus and may result in enhanced expression such as was recently reported for Sindbis virus in a mammalian cell line . The Sf9, Sf21, and High FiveŽ cell lines are suitable for use in expressing recombinant proteins with baculovirus and other insect expression systems (e.g. An insect cell line capable of growth in serum-free media and secretion of high levels of recombinant proteins, including virus-like particles, upon infection with recombinant baculoviruses, is provided. lately the cells started to clump and float after new passages. InsectSelectŽ System). Derived from the ovaries of the fall armyworm moth (Spodoptera frugiperda), these cells are probably related to all insect cell lines in labs worldwide (1). Both primary cell cultures and established lines are used in basic biological studies to reveal how insect cells work. No. Sf9 cells can be cultured as adherent or suspension cells. Most cell lines are now available in serum-free medium that allows higher cell densities (>10 7 cells/ml) to be attained in suspension cultures, hence improving the productivity of the baculovirus expression system. Cat. The insect cell line will provide time and cost savings in analytics and validation, and speed development by circumventing process steps normally required to prove elimination of rhabdovirus. # Product Size Price License Quantity Details; 631411 IPLB-Sf21 Insect Cells: 1 Vial: USD $202.00: IPLB-Sf21 insect cells may be used as a host for propagating the Autographa californica multiple-enveloped nuclear polyhedrosis virus (AcMNPV) and its expression vector derivatives generated from ourBacPAK system. 11605-094 or equivalent). Methods involving clonal selection processes, serum-weaning and recombinant protein secretion selection are used to create the insect lines of the invention. 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